論文の残滓

特許実務に関するあれこれ。何かあれば→ imperatorkm@じーめーる.com

EP Patent status and claim 1 of VILNIUS‘s CRISPR-Cas9 Patents.

執筆用メモ

 

VILNIUS大学の欧州特許、OA苦戦中であったが、1月23日にGrantがでようで。

5者の中で最先でFileしているので、代理人がもっと優秀であれば、結果は違ったかも知れません。明日は我が身…。

 

EP2828386

1 . A in-vitro method for the site-specific modification of a target DNA molecule, the method comprising

     contacting, under suitable conditions,
         a target DNA molecule; and
         an RNA-guided DNA endonuclease comprising at least one RNA sequence and at least one of an RuvC active site motif and an HNH active site motif;

       wherein

       the RNA-guided DNA endonuclease is a Cas9-crRNA complex

       said Cas9-crRNA complex comprising a Cas9, crRNA and tracrRNA
       to result in the target DNA molecule modified in a region that is determined by the complimentary binding of the crRNA sequence to the target DNA molecule,

      the method further comprising assembling the popeptide-polyribonucleotides complex in-vitro by incubating said components of the complex under conditions suitable for complex assembly,

      wherein the target DNA is double stranded or single stranded and wherein a double stranded target DNA contains a proto-spacer adjacent motif.

 

・日米欧共にin vitro assemblyが必須なので、特許としては…